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Lysates were clarified of genomic DNA by passage through QiaShredder spin columns (Qiagen) and heated to 95°C for 5 min prior to separation on a 7.5% polyacrylamide gel.
The cell lysates were clarified of cellular debris by centrifugation (13 200 × g) for 10 min at 4 °C.
Cell lysates were clarified of cellular debris by centrifugation (10000xg) at 4*00BA*C for 10 min, and the supernatants were used in the biochemical assays.
Cell lysates were clarified of cell debris by centrifugation at 14,000x g for 5 min through a QIAshredder spin column assembly (Qiagen, Hamburg, Germany) at 4°C.
The mixture was incubated for 30 min on ice, combined with vortexing every 10 min. Cell lysates were clarified of cell debris by centrifugation at 14.000 × g for 5 min through a QIAshredder spin column assembly (Qiagen) at 4°C.
The mixture was incubated on ice for 30 min, combined with vortexing every 10 min. Cell lysates were clarified of cell debris by centrifugation at 14.000 g for 5 min through a QIAshredder spin column assembly (Qiagen, Hilden, Germany).
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