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Lysates were clarified at 16000 g for 20 min.
Lysates were clarified at 13,000 g for 5 min, and either analyzed immediately or stored at −80 °C.
Lysates were clarified at 18 000×g for 15 min and protein concentration was determined using Pierce BCA Protein Assay Kit Thermo Fisher Scientificc).
The suspensions were clarified at 1.36×103 g for 5 min and the supernatants were dried with nitrogen gas.
Solubilized inclusion bodies were clarified at 15,000×g for 15 min at 4°C and the supernatant was applied to 1 ml HisTrap HP column (GE Healthcare, Sweden).
The cell lysate or homogenized brain lysate were clarified at 12,000 g for 10 min at 4°C, the 3×FLAG-LRRK2 protein was purified using anti-FLAG Affinity Gel according to the manual.
Similar(43)
The homogenate was clarified at 16000 g for 20 min.
Roles and responsibilities should be clarified at the outset.
When asked about the email this week, Dunn told the Guardian: "It was clarified at a later date.
This section should be clarified, at the very least, to bring it into line with the language on page 55.
However, the role of such proteins in ML transport remains to be clarified at the molecular level.
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