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Selected bands were circumscribed from the gels, re-hydrated in 100 μl DEPC-treated water and heated at 50°C for 30 min. Re-amplification reactions included 2 μl of gel band eluate and the anchor and arbitrary primers used in the DDRT-PCR step under the same reaction conditions and cycling parameters described above, excluding the isotope.
Similar(59)
We identified reference barriers by visually inspecting which topographic barriers from the model were circumscribed by the distributions of reference taxa.
Thus, any difficulties that might have arisen from this type of intervention (e.g., non-compliance), were circumscribed to a certain extent.
Their existences were circumscribed by poverty.
All 56 isolates were circumscribed by a 230-km ellipse.
Although the boys' lives seems to be circumscribed by their isolation from the world and their complicity in the chaos of their parents' fraught marriage, change inevitably comes as adolescence steals up on the narrator.
(The right to "bear arms" should also be circumscribed to prevent civilians from owning weapons that are designed purely to kill as many people as possible in a matter of seconds).
Unlike Europeans, whose lives have long been circumscribed by meddling governments and diktats from Brussels, Americans are supposed to be free to choose, for better or for worse.
This shows that the innovative behavior of handling sunflower seeds resulted from individual experience and was circumscribed mostly to males.
Each of the 12 arrays on a slide was circumscribed with a wax border to segregate the arrays from each other.
The game is at its best when you are circumscribed in your actions -- when, for example, you are prohibited from killing anyone or when you must kill someone before he has a chance to press an alarm.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com