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All samples were chilled for 4 h at 5 °C and then frozen to −196 °C.
This study investigated the effect of methanol on sox gene and protein expression in zebrafish embryos (50% epiboly) when they were chilled for 3 h and subsequently warmed and cultured to the hatching stages.
After washing with cold PBS, cells were chilled for 10 min on ice to prevent endocytosis before addition of CHIKV (m.o.i. of 5).
Cells were chilled for 10 min at 4°C and infected with purified MVs at a multiplicity of infection (MOI) of 1 2 plaque forming units (PFU) per cell.
G. m. morsitans were infected with a bloodmeal supplemented with 15 mM GSH; three days post-infection, flies were chilled for 30 min at 4°C then returned to 25°C.
petraea (Plech, Bavaria, Germany) were chilled for more than 2 weeks.
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The bulb must be chilled for about 15 weeks.
The filling can now be chilled for a day or two, or frozen.
Seeds, which should be chilled for six weeks before planting for best germination, are available from Pinetree Garden Seeds, P.O.
The glovebox is chilled for cold drinks; the dashboard cup holder deploys in a delightful kinetic ballet.
The light, somewhat austere 2004 version, harboring hints of tar and anise, is noticeably brightened by being chilled for 10 minutes in the refrigerator.
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