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Library sizes were checked on BioAnalyzer chips (Agilent Technologies, Santa Clara, CA, USA).
RNA degradation and contamination were checked on 1% agarose gels.
Plates were checked on daily basis for growth and nature of microbes.
The PCR products were checked on agarose gels (1%) by electrophoresis.
PCR products were checked on agarose gels.
PCR products were checked on a 0.8% ethidium-bromide gel.
The PCR products were checked on a 2% agarose gel.
The fractions were checked on SDS-PAGE gel and pooled.
Results of PCR amplifications were checked on 1% agarose gels.
The results of the PCR amplifications were checked on agarose gels.
All RNA and cDNA concentrations were checked on a Nanodrop 1000 spectrophotometer.
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