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After 24 h incubation at 37 °C, all plates were checked for growth.
The selected PHA accumulators after Nile blue A staining were checked for growth and PHA production in both nutrient broth (beef extract 0.3%, peptone 0.5%, sodium chloride 0.5%) and cardboard industry effluent.
Cultures on tributyrin, lichenin, chitin and skim milk plates were checked for growth (MM) and zones of clearing around the colonies (MM and ½LB).
Clones were checked for growth defects on a nonfermentable carbon source (YPG 2% glycerol) and absence of mtDNA was confirmed by DAPI staining.
Isolates positive in germ tube assay were checked for growth at 42°C to differentiate C. albicans from C. dubliniensis [ 21].
The plates were checked for growth after 5 8 days of incubation at 37°C both in air and 10% CO2 atmospheres.
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Then the wells were checked for fungal growth using a stereo microscope.
Mutants were checked for known growth defects http://www.broadinstitute.org/annotation/genome/neurospora/MultiHome.html.html
These plates were incubated at 37 °C aerobically and after overnight incubation, they were checked for bacterial growth.
Before use, all strains were checked for purity by growth on peptone-yeast-glucose-starch (PYGS) and reinforced clostridial medium with 5% (w/v) skim milk agar plates incubated under aerobic and anaerobic atmospheres (Carter et al. 2009).
All other strains were kept as spore stocks in Robertson's cooked meat broth (RCMB) at 4 °C, and prior to use were checked for purity by growth on PYGS (peptone, yeast extract, glucose, starch) and Reinforced Clostridial Medium with 5% (w/v) skim milk agar plates incubated under aerobic and anaerobic conditions (Stringer et al. 2009).
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