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We also noted that when both libraries were checked for contamination by WSSV sequence, 167 ESTs were found to be WSSV contaminants in the PmTwI library (E value < 10-25, score > 100), whereas there were no WSSV contaminants in PmTwN.
After analysis of the immune phenotype markers HLA2DR, CD80, and CD83 for DCs and CD3, CD8, and CD56 for CIK cells by flow cytometry, the cultured samples were checked for contamination by bacteria and fungi, and endotoxin levels were less than 5 EU/kg.
Samples were checked for contamination by genotyping them on an optimised panel of 15 microsatellites known to be highly polymorphic within Teno Atlantic salmon using the protocol outlined in Vähä et al. (2008); samples were deemed contaminated if more than two alleles were observed at any locus in an individual.
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All sequences generated in this study were checked for contaminations with BLAST searches [ 89] implemented in the GenBank database on the NCBI webpage (http://blast.ncbi.nlm.nih.gov/Blast.cgi).nih.gov/Blast.cgi
Recently a shipment of her shoes, which are made in Montelupo, Italy, was held up in customs for three weeks because the boxes had been packed in a container of leather pelts, which apparently needed to be checked for contamination.
They did tell Naoto not to eat any more locally produced food, so now he drinks spring water that has been checked for contamination and eats relief supplies delivered from the outside.
Following injection, the skin/mucosa should be checked for contamination.
For each lane a random subsample of 10,000 reads was checked for contamination.
The purity of the chloroplasts was checked for contamination by peroxisomes by testing for catalase activity [ 84].
It was checked for contamination with fat body or hemocyte material by RT-PCR with primers derived from genes exclusively expressed in either of these tissues (P6 and hemese respectively).
Both Vero and HEp-2 cell lines were authenticated by ATCC and were checked for Mycoplasma contamination in our laboratory.
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