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All the tasks were carry out in end expiratory, open glottis apnea in order to avoid variation in autonomic activity due to breathing.
Strand-specific preparation, sequencing and processing of Total and PolyA RNA samples were carry out as previously described [ 32].
Translation, SRDA and homology search in the converted database were carry out using special functions on VBA language for use in MS Excel (see additional file 2).
Real time quantitative PCR assays were carry out in an ABI 7900HT Fast Real-Time PCR instrument (Applied Biosystems, Foster city, CA) using standard PCR conditions.
After four wash steps with PBS-Tween 20 buffer, blocking procedures were carry out for 1 h with 0.1% of bovine serum albumin (BSA) and 0.05% sodium azide in PBS.
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Past drawdowns were carried out in peacetime.
Repairs were carried out following the war.
No executions were carried out in 2014.
Salvage excavations were carried out in 1989.
Similar investigations were carried out in Sweden.
Further repairs were carried out in 1667.
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