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Both were calculated under control conditions and in response to the raclopride challenge.
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Starting with initial concentrations, steady-state values were calculated under acyl-CoA dehydrogenase activity conditions of healthy controls.
Power were calculated under the condition of Minor Allele Frequency (MAF) = 0.273, sample size = 672 (1.03 control per case), type I error = 0.05.
P-values were calculated under this assumption, and adjusted for multiple testing using the false discovery rate controlling procedure.
We used transcript accumulation relative to controls under the ∆∆Ct format to get only positive values, and the carotenoid accumulation levels were calculated relative to controls.
Fold increase in transcript abundance under stress conditions were calculated with respect to their controls.
The fold changes in each infected sample compared to the expression level detected in the corresponding sample under control conditions were calculated using the 2-ΔΔCT method [ 67].
Relative luminescence values were calculated relative to the untreated controls.
Receiver operating characteristic curves and area under curve were calculated.
Areas under the curve (AUCs) were calculated for regions of interest, which were significantly different when compared with controls.
The induction ratios shown in the tables were calculated as the relative increases in expression under Zn deficiency compared to the level of expression under control conditions.
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