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Spot intensities were calculated subsequent to normalization of the spots using the pair-match-based normalization algorithm implemented in the software.
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Model fit was calculated subsequent to correcting for correlated error between 16 of 210 possible item pairs, χ 2 df = 150) = 271.30, p < .01.01
Attack rates (number of subsequent cases of invasive or severe GAS disease divided by number of household contacts, expressed as subsequent cases per 100,000 household contacts) were calculated for subsequent GAS disease among household contacts.
Relative expression levels were calculated with subsequent ΔCT values that were assessed using the comparative CT method with subsequent ΔCT values normalized to the housekeeping genes HPRT and GAPDH.
The load displacement responses of both CHMC and carbon-fiber reinforced epoxy (CF/epoxy) specimens were obtained under various crushing speeds; and crashworthiness parameters, such as the average crushing force and specific energy absorption (SEA), were calculated using subsequent load displacement relationships.
Z-scores based on the UK90 reference data were calculated for subsequent analyses.
Screening intervals were calculated for subsequent screening examinations by taking the period between the current and previous screen.
The number of invasive cells was counted from five random fields from each well and total cells counted for each well were calculated for subsequent statistical analysis.
Positive feeling statements were reversely coded and then the mean scores of the ten items (possible range 1 - 4) were calculated for subsequent analyses to overcome the problems of randomly missing items.
All measurements were taken in duplicate and means were calculated for the subsequent statistical analyses.
All samples were amplified in triplicate and the average CT values were calculated for their subsequent expression analysis.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com