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The unbound secondary detection proteins were removed by two washes with TTBS, while the bound biotinylated secondary detection proteins were bound with a streptavidin-horse radish peroxidase conjugate (SA-HRP) (100 μL/well of a 1 μg/mL stock) for 1 hour at 25°C.
Some were bound with cord.
Her feet, hands and mouth were bound with tape.
His hands, feet and mouth were bound with duct tape.
Their hands, they said, were bound with wire.
Fortunately the books were bound with metal tapes through holes so they could be taken apart...
Farfalle, sun-dried tomatoes and chunks of shrimp were bound with a fine tomato cream sauce.
Investigators said they were bound with chains, repeatedly raped and deprived of food and bathroom facilities.
Fusilli, sweet shrimp and asparagus were bound with a pleasant, light tomato cream sauce.
Fortunately the books were bound with metal tapes through holes so they could be taken apart. . . .
Her legs were bound with black leg restraints before several officers carried her into back of a police van.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com