Sentence examples for were blotted overnight from inspiring English sources

Gels were blotted overnight in 20× SSPE.

The resolved proteins were blotted overnight to a nitrocellulose membrane, and the membranes were blocked in PBS 1× containing 5% NFM (Non-Fat Milk) for at least 1 h.

Membranes were blotted overnight at 4°C with anti-SRF G-20 (1∶200), anti-glyceraldehyde-3-phosphate dehydrogenase (anti-GAPDH; 1∶2000) (Santa Cruz Biotechnology) and anti α-Tubulin (1∶2000) antibodies (Sigma).

Membranes were blotted overnight at 4 °C with the indicated primary antibodies and developed using the HRP substrate western blot detection system (Millipore).

The resolved proteins were blotted overnight onto nitrocellulose membranes, which then were blocked in 1 × PBS containing 5% non-fat milk for at least 1 h.

Gels were blotted overnight onto nylon membrane using a Turbo-Blotter capillary system (Schleicher and Schuell) and 20× SSC transfer buffer.

A nitrocellulose membrane was used to absorb the proteins from the SDS-PAGE and was blotted overnight at 70 mA.

DNA was blotted overnight onto a Gene Screen Plus Hybridization Transfer Membrane (Perkin-Elmer) using the capillary transfer method.

Membranes were blotted with primary antibody overnight at 4°C and washed three times with PBST buffer containing 1× PBS and 0.1% Tween-20 for 10 min each wash.

The separated RNAs were then blotted overnight to Hybond-N+ membrane (AP Biotech) by capillary transfer using 10×SSC [60].

Membranes were then blotted overnight at 4°C in TBST with 3% BSA with the appropriate primary antibodies.