Sentence examples for were blocked overnight from inspiring English sources

Membranes were blocked overnight at 4°C in PBS containing 5% non-fat dry milk and probed with primary antibody (1/1000 dilution) at 4°C overnight.

After protein transfer, NC membranes were blocked overnight at 4 °C with 5%% (W/V) skim milk (DIFCO) in PBS 1×.

Cells were blocked overnight with a solution containing 10% goat serum and 0.3% Triton in PBS.

Gels were blocked overnight in 10% nonfat dry milk in 1× PBS/Tween (0.05%).

Membranes were blocked overnight in Tris-buffered saline containing 0.1% Tween 20 and 3% BSA at 4°C.

Membranes were blocked overnight at 4°C using Rotiblock (Roth, Karlsruhe) before incubation with the primary antibody.

Plates were blocked overnight at 4°C, and washed three times with 150 µl Leptospira Enrichment EMJH (Difco).

The blots were blocked overnight at 4°C in PBS containing 0.1% (v/v) Tween-20 and 5% (w/v) non-fat dry milk powder.

The membranes were blocked overnight at 4°C in TBS containing 0,05% Tween and supplemented with 3% BSA and 3% skimmed milk.

Membranes were blocked overnight in 5% BSA, followed by a three-hour incubation in primary antibodies (0.5 µg/ml) at room temperature.

Membranes were blocked overnight in blocking buffer [PBS supplemented with 1% bovine serum albumin, (BSA; Sigma) and 0.1% Tween-20] at 4°C.