The antibody selection procedure including CAIX-PMPL preparation and characterization, panning, cell-based ELISA screening, and confirmation by flow cytometric analysis as well as DNA sequencing were basically performed following the procedure described previously [38], [39].
All analyses were basically performed using R (ver. 2.14.1) [ 19] and Bioconductor [ 28].
Anesthesia, analgesia, catheter insertions, shock induction, resuscitation schedule, and blood sampling, were basically performed as described previously with slight modifications [ 10].
Fragmentation of cRNA and hybridisation to HuEx1.0 microarrays (Affymetrix, Santa Clara, CA, USA), washing, staining and scanning of the arrays in a GeneArray scanner (Agilent) were basically performed as described previously (Schramm et al, 2005a, 2005b).
Cell size analyses via FSC were basically performed in two different ways as previously described (12): overall cell size distribution, regardless of the different cell cycle phases, was investigated via one-dimensional FSC histograms.
The experiments of ATP/GTP hydrolysis were basically performed as described (Terasaki et al, 2004; Ikeuchi et al, 2005) in a reaction mixture (50 μl) consisting of 100 mM Tris HCl (pH 7.8), 10 mM KCl, 10 mM MgCl2, 10 mM DTT, 0.1 250 μM ATP with 0.25 μl of [α-P]ATP (∼3.7 GBq ml−1, GE Healthcare) or 0.1 250 μM GTP with 0.25 μl of [γ-P]GTP (∼3.7 GBq ml−1, GE Healthcare), and TmcA (50 pmol).
In order to evaluate the performance of SARI image authentication system under common image storage, transmission and processing scenarios, the following test is basically performed from a consumer's perspective.
The sudden descent of a red curtain, from which Ms. Rose emerges to sing the song, is more confusing than effective in a show that is basically performed as a concert, with minimal musical staging (by Chase Brock).
The convolution procedure is basically performed against the connected atoms in both methods.
The well-testing analysis is an inverse solution to the reservoir model identification which is basically performed using the pressure derivative plots.
The embedding operation based on the homomorphic property is basically performed for each element of fingerprint information which will be composed of bit-sequence or spread spectrum sequence, hence each element is separately embedded in its corresponding position.
