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The initial values of the parameters were assigned to zero, i. e. θ0 = [0,0]T.
After normalisation, negative ΔCt values were assigned to zero to reflect their expected copy number state.
All negative kinship values between the individuals were assigned to zero, according to Yu et al. [ 41].
Negative EGP values during the high-dose clamp were assigned to zero as previously described (19, 20).
Samples with three or more missing Fam- KIR3DL1 or Cy5- KIR3DS1 Ct values out of the four well replicates, were assigned to zero copies of KIR3DL1 or KIR3DS1 respectively.
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The participants were assigned to two groups.
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Seventy-two participants were assigned to three groups, each group testing a different evaluation system.
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