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Eventually, 127 primary tumours were assigned a staining score.

In addition, 33 metastatic lesions and 10 local relapses were assigned a staining score.

The immunohistochemical staining results were assigned a maximum score considering both the intensity of staining and the proportion of tumour cells showing unequivocal positive reactions (see Figures 1 3 in the Supplementary Appendix).

cDNA samples were only considered to be from non-degenerate tissue if all haematoxylin and eosin stained sections were assigned a histological grade of degeneration <4.

Slides showing no purple precipitation and only eosin counterstain were assigned a value of zero (0+), whilst cells stained with precipitate were assigned a value between one and four (1+ to 4+).

The BubR1 expression level in lymph follicles, which were equally stained in all samples, was assigned an expression score of 1. Weaker staining was assigned a score of 0, and samples with stronger staining than that in the follicles were assigned a score of 2 (Fig. S1).

Adults were assigned a supporting role.

IHC results were assigned an intensity and percentage score based on intensity of positive staining and number of cells staining, respectively.

Each specimen was assigned a score for staining intensity (values 1 4, with 4 representing the most intense stain) and a score for percentage of positively stained cells (values 0 6, with 6 representing greater than 75%).

Therefore, we first selected an area of tumor cells within each tissue section that showed the most intense cytoplasmic staining with the COX2 antiserum as the "region-of-interest" (ROI), which was assigned a score of 0 (background staining) to 3 (intense staining).

Staining intensity was assigned a score as follows: 0, no staining; 1, weak intensity; 2, moderate intensity; 3, high intensity; and 4, very high intensity.