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All probes with values less than 0.33 were assigned a genotype of B73 and probes with values greater than 0.66 were assigned a genotype of Mo17.
All probes having values of less than 0.33 was assigned a genotype of B73, while probes having values greater than 0.66 were assigned a genotype of Mo17.
Probes that were significantly different (q<0.05) from B73 but not from Mo17 in the RIL hybridizations were assigned a genotype of B (Class I) and probes that were different from Mo17 but not from B73 were assigned a genotype of M (Class II).
To be assigned a genotype a BAC had to have at least 5 probes that were assigned a genotype of B73 or Mo17 and these probes had to exhibit at least 80% genotype agreement within the BAC.
If a SNP was monomorphic in one HEB family but polymorphic in a second family, lines of the first HEB family were assigned a genotype value of 0 to keep a full genotype data set, which is a pre-requisite for the subsequent multiple regression analysis.
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Accounting for obligate missingness, we discarded samples with a genotyping rate of below 89.47% (corresponding to less than 17/19 SNPs being assigned a genotype), and SNPs with (i) a genotyping rate below 90%, (ii) a minor allele frequency (MAF) below 1%, or (iii) a combined sample Hardy Weinberg test p-value below 10-10.
An important final consideration is that material identified in breeding programs can be assigned a genotype.
Each library in the simulated data was assigned a genotype, with a probability proportional to the number of libraries of that genotype in the real data (this proportion was adjusted as each simulated library was assigned a genotype).
SNPs that cannot be assigned a genotype are returned as "no calls" and shared "no calls" for sample pairs were not considered a difference.
After finding variable positions, each individual F2 must be assigned a genotype (homozygote for either allele or heterozygote) at each site (step 3a).
A SNP with a BAF or LRR value not located within close proximity to an allelic cluster cannot be assigned a genotype and is thus labeled a No Call (NC).
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CEO of Professional Science Editing for Scientists @ prosciediting.com