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Kiss1 mRNA levels were assessed using an in situ hybridization (ISH) protocol described by us previously [ 22, 30].
These measurements were assessed using an intrasession intrarater reliability design.
All the participants were assessed using an ad hoc patient's record form.
Data were analysed using Stata 11 and patients were assessed using an intention-to-treat analysis.
Liquid absorption rates were assessed using an optical method and compared to DTPA absorption rates.
Cell viability after incubation with optimized nanoparticles and films were assessed using an MTT biochemical assay.
Models derived from both survey forms were assessed using an independent data set (EVALUATION).
In this study, solubility and metastable zone limit curves were assessed using an agitated and thermostated batch crystallizer.
Calcium and phosphate concentrations in the coating were assessed using an X-ray electron probe analyzer INKA Energy 200 (Oxford Instruments Analytical, UK).
The size distribution and concentration of the nanoparticles in the solution were assessed using an optical particle-size analyzer SALD-71000, Shimadzu, Kyoto, Japan).
Oxygen consumption (VO2) and carbon dioxide production (VCO2) during exercise were assessed using an online gas analyser (Oxycon Pro, Jaeger, Germany).
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