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Systemic diseases were assessed by an internist.
Correlations were assessed by Pearson coefficient.
Intergroup differences were assessed by t test.
The samples were assessed by Salkowski's method.
Knowledge levels were assessed by questionnaire administration.
Subjective symptoms were assessed by questionnaire.
Relationships were assessed by Spearman's r.
Spleen samples were assessed by immunohistochemical staining.
Statistical differences were assessed by t Student.
Differences were assessed by paired t-tests.
Results were assessed by factorial ANOVA.
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