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The samples were assayed using the plaque forming technique.
Residual activities were assayed using pNPαAraf as the substrate at 37 °C for 30 min.
The cells were assayed using a luciferase assay kit 24 h after transfection.
The TsPOx activities were assayed using d-glucose as the substrate.
IL-6 and IFNγ levels were assayed using ELISA preoperatively and at 24 and 48 hours.
24h post-transfection, the cells were assayed using a luciferase assay kit (Promega, Madison, WI, USA).
Serum levels of all metabolites were assayed using liquid chromatography/tandem mass spectrometry (LC-MS/MS).
At 24 h after transfection, cells were assayed using luciferase assay kit (Promega).
Firefly luciferase and Renilla luciferase activities were assayed using the Dual Luciferase Reporter Assay Kit (Promega).
24 h post-transfection, the cells were assayed using a luciferase assay kit.
At 24 h post-transfection, the cells were assayed using a luciferase assay kit.
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