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These functional properties were ascertained based on earlier published studies and by extensive bioinformatics analyses.
All subjects were ascertained based on a clinical diagnosis of DMD supported by the identification of a mutation in the DMD gene.
Second-degree relatives were ascertained based on the aforementioned relationships.
Comorbidities were ascertained based on the presence of relevant diagnosis codes during the history period.
Comorbidities were ascertained based on the presence of diagnoses during the six-month pre-index period.
Improvements in cough were ascertained based on self-reported assessments of cough during followup visits.
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Receipt of chemotherapy was ascertained based on the presence of one or more claims for a chemotherapy drug or administration, which were identified using HCPCS and ICD-9-CM codes in the healthcare claims database.
For example, only one hybrid (field identification number 1065) from site JR could be ascertained based on morphological criteria during field sampling and our genetic analysis recognized it to be an F2.
Nano-indentation was used to determine the Young's modulus and hardness of the coatings, while microtensile tests were used to introduce controlled strains in the coatings through the cp Ti and TiAl6V4 alloy substrates, from which the strength, fracture toughness and adhesion behaviour could be ascertained based on multiple cracking and delamination events.
This presupposes that the stability of the well system can be ascertained based on advance knowledge of the wellbore/rock behaviour derived from established rock mechanical and failure models.
While distant, sequence-based relatives of caspase-like proteins (e.g., meta- and paracaspases) have been recognized in silico in various unicellular organisms, however, bacterial orthologs have yet to be ascertained based on DNA sequence (Vercammen et al. 2007).
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