The optimal ERα staining (low background, strong nuclear staining, no cytoplasmic staining) was determined to be as indicated below.
RU was added at different concentrations, cells were harvested 4 days later, and apoptotic/necrotic versus total cells were counted as indicated below.
Due to insufficient cost data related to control of lead hazards, only preliminary estimates of cost incurred by pollution control were performed, as indicated below.
The insulin-releasing activities of fractions were determined as indicated below.
Kidney extracts were obtained as indicated below.
Seven days after injection, animals were perfused as indicated below.
ARPE-19 cells were used as indicated below.
BamH I and Hind III recognition sequences were added as indicated below.
The most appropriate temperature, food source,and concentration method for growth of Acanthamoeba castellanii (ATCC 30010) type strain were determined as indicated below.
After reaching different optical densities at 600 nm (OD600) (0.5, 0.5, and 0.8), cultures were induced with nisin (Danisco, Grindsted, Denmark) at different concentrations (0, 1, 10, 50, 100, 200, and 500 ng/mL) and cytoplasmic and cell wall protein extracts were prepared as indicated below every hour until 6 h after induction [ 20].
