The PCR conditions were as following: 95°C for 10 min, followed by 94°C for 45 s, 58°C for 60 s, and 72°C for 60 s for a total of 31 cycles.
Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted using 5 ng cDNA for all genes of interest and 1 ng cDNA for 18S rRNA and the cycle conditions were as following: 2 min at 50°C, 10 min at 95°C, followed by 40 cycles of 15 sec at 90°C and 1 min at 60°C.
In terms of specialties, in Greece the largest proportion of the participants were pathologists (14.1%), followed by paediatricians (10.3%) and gynaecologists (9.1%), whilst in Cyprus the percentages were as following: paediatricians (17.1%), pathologists (9.6%), gynaecologists and surgeons (9.1%).
The objectives of this research were as following; identifying the consumer's needs and want that lead to their selection principles.
The conditions were as following: 1) continuous cutting: turning of annealed 1040 steel (HB 220); 2) interrupted cutting: turning of 4340 steel (HRC 40); 3) end milling: ball nose end milling of hardened H13 steel (HRC 50).
Cases that have been discussed were as following: higher and professional education, research, professional projects in the field of engineering management, mechatronics, robotics, quality management, IT adoption in developing countries, mobility, internationalisation, employability, etc.
The key conditions for HR-ICP-MS analysis were as following: the plasma power was 1350 W, the flow rate of cool gas, aux gas and sample gas was 16.86, 0.99 and 1.123 L/min respectively.
The results show that the optimized conditions of microalgal bead preparation were as following: sodium alginate concentration of 4% (w/v), CaCl2 concentration of 2% (w/v), bead size of 4.7 mm and initial algal density of 0.2 × 106 cells mL−1.
The procedures were as following: 1) to examine and analyze the principles and theories 2) to explore the context concerning knowledge construction and problem solving transfer, and 3) to synthesize theoretical framework and designing framework of the multimedia learning environment to enhance problem solving transfer.
The experimental variable was the lesson plan of the maritime medicine nursing practicum using the research-based instruction while the dependent variables used in the study were as following: 1) the nursing students' ability to conduct researches and 2) the knowledge relating to researching.
