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The settings in MrBayes were as above, but the number of MCMC generations was set to 30 million.
Oligonucleotides tested were as above and crHyb.duplex.mut 5′-rAGC ACT GCA CGC CGrU rAGG rUCT rArArA ATT ACG ATA CGA CAA AAC-3′.
Thermocycling conditions were as above, except that 25 cycles were used.
Thermocycling conditions were as above, except for an annealing temperature of 65°C.
The reaction conditions were as above, but without fluorescently labeled dUTPs in reactions 1 and 2, and with primer concentrations as noted in Table S2.
In the second experiment, the CIE coordinates of the blue standard were as above; the less-saturated, equiluminant blue were Y = 2.6; x = 0.178; y = 0.150; and the equally-bright blue annulus co-ordinates were Y = 4.1; x = 0.178; y = 0.150.
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Secondary antibody was as above described.
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