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Additionally, 40 unigenes were annotated to be involved in the biosynthetic steps from GGPP to taxol within 'diterpenoid biosynthesis' pathway.
One potential and partial taxol biosynthesis pathway in C. cladosporioides MD2 was speculated, and dozens of unigenes were annotated to be involved in taxol biosynthesis.
Based on the KEGG analysis, 40 unigenes in the C. cladosporioides MD2 transcriptome were annotated to be related to the fungal taxol biosynthesis, and were homologous to the genes for TS, T5αH, T13αH, and TBT, respectively.
In the S. gregaria database several ESTs were annotated to be involved in GPCR signaling pathways (GO: 0007186).
Many of these genes were annotated to be involved in efflux, transport, iron uptake, signal transduction via methyl-accepting chemotaxis proteins, flagella, pilus, and fimbriae biosynthesis, or have an unknown function.
In total we found: 152 proteins containing the RR motif of which 84 had one or more transmembrane domains; 191 proteins containing the KK motif, of which 109 had one or more transmembrane domain and 34 proteins containing the XDEL motif, of which 24 were annotated to be found in the ER/SR lumen (Figure 7B).
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All of these seven proteins are annotated to be bound by MSH2, RFC4 and MSH6.
The Gene Ontology (GO) analysis revealed that AtWAKL10 is annotated to be part of the endomembrane system, have kinase activity and function in protein amino acid phosphorylation.
According to the information provided for the OEP9 gene locus at GenBank and TAIR, the deduced protein is annotated to be of unknown function and possess no putative targeting signal motifs.
Clo1313_0124 is annotated to be ferredoxin dependent.
Among them, 2,240 had been annotated to be one single protein class while the rest 648 targets have been annotated to be multiple protein classes.
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