At 2 h after the injection, mice were anesthetized via 2% isoflurane inhalation.
Six, male, retired breeder, Sprague-Dawley rats aged 8 months were anesthetized via isoflurane inhalation.
At 1, 2, and 4 h post injection, mice were anesthetized via 2% isoflurane inhalation.
At the definite time, mice were anesthetized via intraperitoneal injection of 150 μL 4% chloral hydrate.
Rats were anesthetized via a nose mask with 4% and 2% isoflurane in oxygen for induction and maintenance, respectively, at a rate of 1 L·min−1.
At 3 h post injection, mice were anesthetized via 2%% isoflurane inhalation and scanned via NanoSPECT/CT (Bioscan, Washington, DC) for 20 min.
Adult male Sprague-Dawley rats (n = 8) weighing 590 ± 19.2 g were anesthetized via an intraperitoneal injection of ketamine/xylazine (90 and 10 mg/kg, respectively) dosed at 0.1 mg/kg of ketamine before surgery as well as during the experiment to provide adequate anesthesia.
Six male Wistar rats (weight: mean, 530 g; range, 480 to 560 g; Charles River Breeding Laboratories, Yokohama, Japan) were anesthetized via an intraperitoneal injection of sodium pentobarbital (30 to 40 mg/kg) (Somnopentil, Schering-Plough Animal Health, Omaha, NE, USA).
