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Stable cell pools were analysed directly after outgrowth in selection medium.
In addition, the wood samples as well as the eluates were shipped to and from ECN Petten, whereas the wood samples were stored under controlled conditions and the eluates were analysed directly after sampling at BAM.
As detected using flow cytometry, more cytokine-producing T-cell subsets were observed in granuloma-derived leukocytes that were analysed directly ex vivo than in the in vitro-restimulated cells from the peripheral blood and skin-draining lymph nodes (dLNs).
In addition the results agreed very well with those obtained when such duplexes were analysed directly as bimolecular complexes, i.e. when the target, modified with a biotin, was directly immobilized onto a streptavidin sensor chip surface rather than captured by an anchor.
Levels of free ascorbate were analysed directly.
Plasma glucose (HemoCue), triglycerides and lipids (ADVIA 1650) were analysed directly after sample collection and LDL-cholesterol was calculated using Friedewalds formula [25].
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Digests were stopped by addition of trifluoro acetic acid (TFA) to approx. pH 3. 10% of the peptide mixture was analysed directly, the remaining 90% were enriched for phosphopeptides by the use of TiO2 as described in [ 48].
Mononuclear fractions were isolated and one sample was analysed directly and the other was supplemented with 'freezing medium' RPMI-16400 medium with -glutamine (Gibco, Breda, The Netherlands) containing 10% dimethylsulphoxide, (DMSO; Merck, Darmstadt, Germany) and 20% fetal calf serum (Gibco) and stored in liquid nitrogen.
The latter were included to allow the singleton loci to be analysed and directly compared with conserved paralogs.
Leaves directly treated with the resistance inducer were analysed to study local effects of T39-induced resistance, and untreated plants were used as control.
Inorganic components were analysed.
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