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Suspension cells were alternatively analyzed by metabolic activity measurements with the XTT cell proliferation kit II (Roche, Mannheim, Germany).
Non-adherent U-937 cells were alternatively analyzed for their ability to form colonies in soft agarose by overlaying them onto 2 ml of 0.4% w/v Sea Plaque agarose (Cambrex, East Rutherford, NJ, USA) on top of 3 ml of a 1% w/v peqGOLD agarose underlayer (PeqLab, Erlangen, Germany), both in complete medium.
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As these genes involved in alternate splicing were temperature-regulated, transcripts of selected SR genes that are known to be alternatively spliced [ 69, 73] were analyzed to explore the influence of temperature on their splicing patterns (Additional file 2: Figure S4).
Alternatively, data were analyzed per 'synapse', that is a reconstructed region of a synapse around the dense projection, for which only one or two events occur such invaginations or the presence of large vesicles.
Alternatively, data were analyzed based on absence/presence.
Serum samples from patients with and without VTE were analyzed alternatively after random selection of run order of each group.
Alternatively, cells were analyzed by time-lapse microscopy using Application Solution Multi-Dimensional Workstation (Leica Microsystems, Mannheim, Germany).
Alternatively, they were analyzed with a Mann–Whitney rank sum test, 1-way ANOVA on ranks, or Spearman's rank correlation coefficient.
Alternatively, images were imported and analyzed on Image-Pro Analyzer software as described above.
Alternatively, lipid extracts were analyzed by MS and MS/MS in an ESI-Q-Tof Micro (Waters), in positive ion mode.
Alternatively the proteins were blotted and analyzed using specific antibodies (NonO: α-p54nrb, mouse IgG1, BD Biosciences/SFPQ: α-PSF (H-80), sc-28730, Santa Cruz).
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