Although the alignment shifting method employed by the S-series had no significant effect on tuning the activation color (tuning range of only 40 nm, emission changing from 630 to 670 nm, Supporting Information Figures S1 and S3), we noticed the emission peaks (under 580 nm excitation) migrated in a periodic fashion as T or A was alternatively added to the 3′ side of the linker.
The AIE of the organogel could be controlled "on-off-on" by alternatively adding F− and H+.
Alternatively, cytokines were added to confluent fibroblast monolayers, and cells were washed with PBS after 96 hours and incubated with 200 μl PBS containing 3 mM GlyPro- p-nitroanilide.
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Alternatively, when exogenous proteins were added, cells were decidualized with E2 (10−8 M; Sigma) and MPA (10−7M; Sigma) for 8 days.
In conclusion, the maximum polysaccharides conversion yield (12.4%%) was obtained for MIX A. The enzymes rCelStrep, rPoAbf or its evolved variant rPoAbf F435Y/Y446F were added alternatively or in combination to 15 mg/g glucan of MIX 1 (60 % Cellic® CTec3 and 40%% Cellic® HTec3 from Novozymes) for the hydrolysis reactions of pretreated NW.
Moreover, rCelStrep, rPoAbf or rPoAbf F435Y/Y446F were added alternatively or in combination to the MIX 1 containing 15 mg/g glucan of commercial preparation mix (60%% Cellic® CTec3 and 40%% Cellic® HTec3 from Novozymes).
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Alternatively, compounds are added at supra-pharmacologic doses and their metabolic products are found to be ligands [10].
