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Postovulatory follicles (POFs) were also counted using the PD method.
Spots were also counted using an AID ELISPOT Reader (ER).
Tyrosine-hydroxylase-immunoreactive cell bodies in different planes in the images of tyrosine hydroxylase staining were also counted using the cell counter plugin.
After 10 14 days of incubation, spheres were consolidated into a 96-well plate and imaged on an EVOS fluorescence microscope (AMG & Life Tech) after DAPI staining and they were also counted using GelCount™ (Oxford Optronix) following iodonitratetrazolium chloride staining.
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Total cell number was also counted using a hemocytometer.
The sperm number in the uterine flushing from each of the females mated with a subset of males in the fertility test was also counted using a hemocytometer.
Unused pills were also counted.
The platelet population in the blood samples after lysis of the red blood cells, was also counted manually using phase contrast microscopy in a Neubauer chamber.
The dynamic of substitution was also counted analytically using the equations describing the first order reaction rate.
Read counts were also counts per million (cpm) normalized using EdgeR (v3.4.2) package and then pre-ranked using Log2 Fold Change.
The number of avoidant/cessation behaviours associated with each pack was also counted and paired t tests were used to test for differences in the mean number of actions taken with the Kerrods pack versus their own pack.
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CEO of Professional Science Editing for Scientists @ prosciediting.com