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MM cells were allowed to migrate for 24 h; thereafter, migrating cells were collected and stained with CD138.
Cells were allowed to migrate for 24 h; the gap area was then photographed and migration distances were measured.
The cells were allowed to migrate for 90 min in a humidified incubator (5%% CO2) at 37 °C.
Cells were allowed to migrate overnight.
The larvae were allowed to migrate for 3 minutes.
Cells were allowed to migrate through the membrane filter for 24h at 37°C, 5% CO2.
Cells were allowed to migrate at 37°C in a cell culture incubator for 18 h.
Over the next 24 hrs cells were allowed to migrate into this denuded area.
Synchronized VSMCs were allowed to migrate for 5 hours at 37°C.
The cells were allowed to migrate for 18 hours in serum free medium at 37°C.
The plate was placed in total darkness and the larvae were allowed to migrate for 3 minutes for the control.
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