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Egg laying & feeding: Female beetles were allowed to lay eggs and to feed (leaf material sampled at 48 h and 72 h after egg deposition).
"[My foster parents' own children] were allowed to lay wherever they wanted, and we couldn't access the full house, and slept in the basement".
Before collection, adults were allowed to lay for 2 hours.
Ten gravid adult animals were allowed to lay eggs for one hour and picked off.
For the learning assays, flies were allowed to lay eggs for two days.
The mothers were allowed to lay eggs for 24 hours, and were then removed.
Gravid adults were allowed to lay eggs for several hours, then removed.
They were allowed to lay eggs on cabbage plants (B. oleracea var. capitata cv Shikidori) in a cage.
Gravid adults were allowed to lay eggs on RNAi-expressing lawns of bacteria for 6-12 hours.
The fully mated females were allowed to lay eggs for the same period (∼1 day) on the standard medium.
5 7 gravid rrf-3 hermaphrodites were allowed to lay eggs on these plates for one day and removed afterwards.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com