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To examine the phylogenetic relationship between NLL and sequenced species, the 13895 quality-screened BESs were aligned (via BLASTn) to the NCBI Nucleotide database and the probable phylogenetic distribution of these hits was visualised with MEGAN [ 39, 40].
These sequences were aligned via their encoded amino acid sequences using the Transalign program (kindly supplied by Georg Weiller) and CLUSTALX [18] with default parameters, and gave an alignment with 720 nucleotides and gaps (240 codons); the alignment of the cCPs of these 'outgroup potyviruses' will be provided on request.
The surfaces were aligned via spherical registration to the Freesurfer average atlas (Fischl et al., 1999).
The repeat families were aligned via ClustalW version 2.0.12, and the RIP index was calculated using RIPCAL [ 79].
The surfaces were aligned via spherical registration to the Freesurfer average atlas (fsaverage, Fischl et al. 1999).
The two laser beams were aligned via a beam splitter such that they illuminated the same surface area of the sample.
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For genes too divergent to be aligned via Maximum Likelihood method (coat and tail genes), translated protein alignments were used to construct Neighbor-Joining trees in Unipro UGENE using the following parameters; a distance matrix model of F84, transition/transversion ratio of 2.0, and a bootstrap (replicate) value of 1000.
TBSS (Tract-Based Spatial Statistics) [29] was then applied and individual whole-brain FA data was aligned via non-linear registration with the most representative subject's brain space and then affine-aligned into MNI152 standard space.
The CT data obtained during t 1 and t 2 are aligned via the proposed registration algorithm (steps A and B) to the CT images acquired at t 3. The resulting DF are then applied to the corresponding PET images to yield co-registered longitudinal PET data (steps C and D).
And though it appears that the two families had at one point been aligned (via a crack ring circa 2002), the subsequent murder of a Marriott made it clear this alliance was no more.
We and others have previously proposed homology models of A3G in which the N- and C-terminal CDA domains are aligned via interactions involving β2 to form a continuous β-sheet as observed in the crystal structure of the closely related A2 protein [12], [16], [35].
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