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The 18 sequences generated were aligned together with reference sequences downloaded from GenBank using the ClustalX program [18].
The sequences obtained here were aligned together with HCV subtype reference sequences using CLUSTAL W program implemented in MEGA 4.0.
Genotype-derived amino acid sequences corresponding to the NS5BΔ21 used in this study (named G1b, G2a, G3a, G4d and G5a) were aligned together with consensus sequences from each genotype (named CON_ genotype) (Fig. 1).
All data sets were aligned together as a single batch using MultiAlign, and centered to the most complete data set using the C. crescentus accurate mass tag (AMT) database [68].
The selected sequences were aligned together and with an out-group comprising sequences of the related WD40 domain protein Lis1, using CLUSTALW in BioEdit and corrected by eye (Supplementary Figures S1 and S2).
For identification and the phylogenetic analysis of UCP candidates, the deduced amino acid sequences of zebrafish UCPs were aligned together with all known UCP protein sequences available in public databases using ClustalX (National Center for Biotechnology Information, Bethesda, MD, USA) and subjected to phylogenetic inferences using the Neighbor-joining (NJ) method.
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To accomplish this, separate alignments are aligned together while preserving the columns in the individual alignments.
Next the blue sub-alignments are aligned together.
To identify which genomes could be aligned together, we built a composite likelihood distance matrix based on the concatenated alignment of protein-coding genes cox1, cox2, cox3, atp6, atp8, and atp9.
These octahedral particles are aligned together in the form of long cylinder.
By properly designing precoding, the desired messages can be aligned together and therefore inter-stream interference can be canceled when the perfect channel state information (CSI) is available.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com