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After the war it lacked the funds and political will to maintain control of colonial possessions that were agitating, with increasing violence, for independence.
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For example, wild-type α-synuclein, which is the main constituent of the Lewy bodies found in Parkinson's disease, took between three and nine weeks to form fibrils when incubated at 37°C without agitation [43], but fibrillation was complete after 36 48 hr when the solutions were agitated with a Teflon stir bar or with a Teflon sphere in a microplate [28], [44].
During processing, the suspensions were agitated with a magnetic stirrer.
The pH values of granular samples were more than 5. Accordingly, specimens were agitated with glacial acetic acid buffer solution #B (pH 2.88 ± 0.05) for 18 h at a temperature of 25 °C maintaining a 1 10 sample-to-fluid ratio at a frequency of 130 r/min.
While the conservative members were agitated with Democrats over the issue, they became more heated when discussing McConnell.
Thymi were agitated with a magnetic stirrer at 4°C for 20 minutes to remove a large fraction of thymocytes.
In incubations for as long as one week, amyloid formation was observed only when the solutions were agitated with a Teflon ball.
Then samples were agitated with 1,200 rpm at 4 °C for 30 min (Thermomixer Comfort, Eppendorf, Germany).
Crude soluble cell extracts were agitated with 3 mL (bed volume) of Ni-NTA resin equilibrated with the buffer for 1 h at 4 °C.
A 300 μM aSyn solution in 50 mM Tris HCl, 150 mM NaCl, and 3 mM NaN3 (pH 7.4) were agitated with 5 mM dopamine in a 0.5 ml reaction volume for 72 hr at 37°C, 1,400 rpm.
The changes proposed are related to the way in which the system is agitated: with magnetic or bubble stirring; or the time during the agitation is applied to the system: along all the determination or only during a specific period of time.
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CEO of Professional Science Editing for Scientists @ prosciediting.com