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LDPE films were again washed with sterile Milli-Q water.
Before AFM and SICM experiments, the treated cell samples were again washed three times with PBS.
The fixed scaffolds were again washed three times with distilled water for 10 min.
Fixed bacterial pellet were again washed with phosphate buffer saline (PBS) at 5000 rpm for 5 min.
Wells were again washed and ABTS substrate was added.
Bead aliquots were again washed and resuspended in ddH2O.
The sections were again washed and incubated with FITC-Streptavidin for 30 min at room temperature.
The plates were again washed and incubated for 1 h at 37°C with secondary antibody.
Sections were again washed in PBS-Tx, and then mounted onto Superfrost plus microscope slides (Fisherbrand).
After one week, the worms were again washed and cycled through fresh soil for another week.
Cells were again washed with ddH2O, and treated with 10% glacial acetic acid.
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