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After the collection of data, the PEEP was decreased to 17 cmH2O and data were again collected as described above.
Then, culture supernatants were again collected for cytokine measurements and macrophages were scraped and lysed in 1 % sodium dodecyl sulfate (SDS, Sigma Aldrich) in PBS using a 23G needle (B. Braun, Melsungen, Germany) to release phagocytosed mycobacteria.
The filter was replaced with a λ compensator and images were again collected through the same 90° span.
The media were again collected after 5 hours, speed vacuumed overnight, and analyzed for ACTH and TRH peptides by radioimmunoassay (RIA).
Cells were again collected, washed twice with PBS, resuspended in 100 µL PBS and incubated with 3 µL of FITC-conjugated anti-goat Ig secondary for 10 minutes at 4°C in the dark.
The supernatant fractions were again collected.
Similar(33)
The culture medium was again collected and sterilized.
When children were 42 months of age, EEG was again collected from children in the care as usual group (CAUG) and foster care group (FCG).
After 5 days of exposure to P. aeruginosa, stool was again collected, homogenized in 1 mL 1% protease peptone, serially diluted in 1% proteose peptone and dilutions plated on TSA and LB agar (with 0.015 mg gentamicin/mL (Research Product International, Mt. Prospect, IL) for PA14 transposon insertion mutants) to measure levels of GI colonization by P. aeruginosa.
Saliva was again collected by passive drool.
Serum was again collected from the mother at the last antenatal examination (34th week of pregnancy).
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