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PBMC were adhered for 2 h at 37°C in 24-well plates.
In brief, single cells obtained from passage 1 (P1) neurospheres were infected o/n by incubation with diluted (1 5) supernatants of shRNA retroviruses produced in Plat-E cells; after 5 days, the clones were dissociated and single cells were adhered for 10 min to a Matrigel substrate and stained.
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The curves in Fig. 4A separate the region where more than 50% of cells are adhered (inside each curve) from the region where less than 50% are adhered for three different scenarios.
Further guidance on NTIDs was adhered for cyclosporine, tacrolimus, and mycophenolate mofetil [ 23].
(D ) Immunoblot analysis of SAG1-HA distribution after differential centrifugation of medium from SAG1-C65-HA and hap2 gametes that had been adhering for 3 hr.
STROBE guidelines for observational studies were adhered to for this study, Patient demographics are displayed in Table 1.
The consolidated criteria for reporting qualitative research (COREQ) were adhered to for all interview and focus group activities.
16 Original pathology reports were adhered to for the classification of metastatic lymph node involvement.
Institutional protocols and guidelines were adhered to for the delivery of anesthesia and analgesia.
All research protocols were approved by Colorado State University's IACUC, and regulations concerning the animal use were adhered to for these experiments.
This process supported the principles outlined in the RATS guideline, and which were adhered to for quality reporting of the study [ 43].
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