Sentence examples for were additionally washed from inspiring English sources

Samples were additionally washed or washed/frozen.

Roots were additionally washed in slightly acidic (4% HCl) milliQ water for 10 min and then rinsed three times in milliQ water.

Blots with a higher background were additionally washed once at 50°C and re-exposed.

For protein identification, the plugs were additionally washed in 50 mM ammonium bicarbonate and incubated in 10 mM DTT at 37°C.

After centrifugation (20,000  g, 15 min, 4 °C) inclusion bodies were additionally washed in buffer W2 (20 mM Tris, pH 7.0, 0.5 % Triton X-100, 1 mM EDTA, 0.1%% β-mercapto-ethanol) and finally in buffer W3 (50 mM Tris, pH 7.0, 1 mM EDTA, 0.1%% β-mercapto-ethanol).

For mass spectroscopy, the immunoprecipitation was performed as described with the following changes: after washing with buffer A, the beads were additionally washed with 100 mM Tris-HCl pH 8.5 and the bound proteins were eluted by incubating with 8 M urea in 100 mM Tris-HCl pH 8.5 for 15 min at 37°C.

After 2 h of reaction, the resin was additionally washed (two times for 10 min) with 20%% piperidine in DMF to remove the phenyl esters formed by the acylation of the CF phenolic hydroxyls.

The sample solutions were diluted (100 1000 times) immediately prior to deposition on the freshly cleaved mica surface and incubated for <1 min. Depending on the dilution, the surface <span class="lh lhl">was additionally washed with a drop of DI water or dried under an argon atmosphere right away.

To prevent pressure artefacts, brains were additionally postfixed overnight within the skull at 4°C, washed and cryoprotected in 20% sucrose in PB.

For measurement of IgA, plates were additionally blocked with 2% BSA for 1 hr followed by three washes with PBST.

For integrin α-4 staining, cells were additionally incubated with a FITC-labelled secondary Ab against mouse IgG (R&D, Karlsruhe, Germany) after a washing step.