This inhibitory effect was not observed when the cells were additionally treated with LH.
Groups I and III were additionally treated with 60 kHz ultrasound (time average acoustic intensity 0.14 W/cm2, duty cycle 50%).
From day 14 of culture, subsets of pellets were additionally treated with one of the four PTHrP isoforms (1-34, 1-86, 7-34, and 107-139) at 100 nM.
For group two, before deposition of HfO2 thin films, Si wafers were additionally treated by remote O2 plasma for 1 min.
The method was not applicable for all of the six waste water samples (clarified sewage), though they were additionally treated with non-polar RP C18 cartridges.
Patients in the test group received an identical treatment as patients in the control group, but were additionally treated with the herbal medicine Canephron®N (2 tablets TID for 3 months).
Two subjects were additionally treated with insulin.
The OB/D subjects were all treated with metformin and 2 of these subjects were additionally treated with insulin.
In some experiments as indicated, cells were additionally treated with bovine TSH (TSHβ) (Sigma) to test its effect on gene expression.
In order to characterize the cellular mechanisms by which UCB can affect the induction of LTP and LTD, slice cultures were additionally treated, in some experiments, with calpain inhibitors, NMDA receptor antagonist, or L-type voltage-gated Ca2+ channel blocker.
All RNA samples were additionally treated with 2 U of DNase I (Promega) at 37°C for 30 min. To stop DNase I activity, DNase I inhibitor (Promega) was added to the reaction tube.
