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All isolates were additionally grown in MH-2 without antibiotics.
Akata cell subclones; EBV-positive AKBM and EBV-negative AK31 (Ressing et al., 2005) were cultured in R10 medium (RPMI + 10% heat-inactivated FCS (PAA) + 50 U/ml penicillin/streptomycin); AKBM cells were additionally grown in selection medium containing hygromycin (300 μg/ml).
For the whole transcriptome sample, C. glutamicum cells were additionally grown in shaking flasks in defined CGXII medium and subjected to different stresses at an OD600 nm of 10. High salt stress: addition of sodium chloride to a final concentration of 10%.
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39 There is additionally a growing literature base of potential system interventions to support the cognitive work of diagnosis that will likely offer new ideas and strategies to teach for future improvement.
To better understand the HS action, the transcript accumulation of selected genes encoding enzymes involved in nitrogen assimilation and Krebs cycle was additionally evaluated in seedlings grown for 2 weeks under nitrogen (N) sufficient condition and N deprivation.
Land availability within the UK for growing bioenergy crops is, additionally, a big issue within the bioenergy field.
That is, additionally, absurd.
The microalgae can additionally be grown heterotrophically and mixotrophically, but on a smaller bench- or pilot-scale.
Additionally, growing perennial grasses needs almost no pesticide, which is helpful for the water quality improvement (Hoekman et al. 2018).
Additionally, cells were grown at 30°C to minimise any TS-effect at this stage.
Additionally, crystals were grown for a 286 nucleotide long variant of the Clostridium botulinum intron.
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