Sentence examples for were additionally amplified from inspiring English sources

For E. cordatum and P. lividus, all loci were additionally amplified with fixed annealing temperatures (named EE-GP in Table 1).

Positive samples were additionally amplified by RT-PCR in the open reading frame 1 (ORF1) according to [ 27], and in the junction gene region between ORF1 and ORF2 using the One-Step RT-PCR Kit (Qiagen, Hilden, Germany).

For selected individuals representing the major evolutionary lineages inferred in the previous analysis, a 362 bp fragment of the large subunit mitochondrial ribosomal gene (16S) and 509 bp of the internal transcribed spacer 1 (ITS-1) from the nuclear ribosomal cluster were additionally amplified and sequenced.

The link with hypercalcaemia is based on the finding that, in particular in combination with vitamin D analogues, the calcification process can be additionally amplified in the vascular system.

Furthermore, an increase of glycolysis can be additionally amplified by endogenous secretion of catecholamines, due to stress during hypoxia.

The D2/D3 expansion segment of the 28S rRNA gene (~600 bp) was additionally amplified from all specimens using primers D2A and D3B [ 28].

The D2/D3 expansion segment of the 28 S rRNA gene (~600 bp) was additionally amplified from all specimens using primers D2Ab and D3B [ 50].

For a subset of individuals (see below), the internal transcribed spacer 1 (ITS-1) from the nuclear ribosomal cluster was additionally amplified and sequenced.

The genomic region covering exon 7 of the MDM4 gene was additionally amplified in 140 breast cancer and 200 control samples to allow for a subsequent restriction-enzyme based screening of the D153G mutation using BbsI (New England BioLabs).

The genomic region covering exon 1 of the MDM4 gene was additionally amplified in 133 breast cancer and 136 control samples to allow for a subsequent restriction-enzyme based screening of the c.-103 T/C variant using MboI (New England BioLabs).

Activated chondrocytes are believed to additionally amplify the proinflammatory cascade of joint destruction through further release of cytokines (such as IL-1, IL-6, and TNF) [ 10, 31, 32].