Sentence examples for were added twice from inspiring English sources

Pre-cooled PBS were added twice to wash the cells.

Firstly, reporter molecules of the PBAT were added twice, which is embedded in the gold core with the reduction of tetrachloroaurate and then PBAT is conjugated again on disperse gold nanoparticles (PBAT-Au).

Fresh twigs were added twice per week.

Growth factors (EGF, bFGF) were added twice per week.

Briefly, cell suspension (100 µl, 1500 CHO cells per well) were seeded in DMEM plus 10 % FCS in 96-well microtiter plate at 37°C. 10 µl of peptides were added twice to the cells after 24 and 48 hours of cell culture to obtain concentrations of 1, 10, 20 and 50 µM and the toxicity was evaluated 48 after treatment.

Fresh aliquots of growth factors were added twice a week.

Indented names of groups in the "Adherents" column are subcategories of the groups above them and are also counted in these unindented totals, so they should not be added twice into the column total.

Indented names of groups in the first, "Adherents," column are subcategories of the groups above them and are also counted in these unindented totals, so they should not be added twice into the column total.

The radionuclide was added twice.

5 ml of test solution was added twice daily to the petridish for 6 days.

The water was totally renewed every 2 days, and food was added twice a day (morning and evening) at a rate calculated by the method mentioned above.