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The particles (1011) were added to either the CHAPS lysis buffer or to the JAWS II lysate for 24 h.
Radioactive solutions of 223Ra, 188Re, or 99mTc were added to either plasmid DNA or to PC Cl3 cells in the absence or presence of DMSO.
Lesser inhibitory effects were found in when the MAPK inhibitor, PD98059 and of the PI3K inhibitor, wortmannin were added to either P or MPA (Fig. 10A B).
Aos1/Uba2, Ubc9 (2 µl each) and SUMO-1 (10 pmol, 2 µl) were added to either recombinant human CBS (8 pmol) or recombinant human CSE (12 pmol) in the presence or absence of purified hPc2 (2 pmol) in the commercially supplied sumoylation buffer to a final volume of 22.5 µl.
Expression is given as the relative message level in mutant strains compared to the WT message levels, which were set at 1. Streptococci were grown to mid-log phase and 0.5 ml of washed bacteria were added to either 0.5 ml of fresh, heparinized plasma from non-immune donors or to 0.1%BSA in PBS as a control, incubated for 10 min at 37°C and washed.
To improve the expression of soluble protein, 1/100 (v/v) of the antibiotic-selected cultures were added to either the Overnight Express™ Instant TB Medium (Novagen, USA) autoinduction system and allowed to grow to an OD600nm≥1.5 at room temperature or added to LB medium without antibiotics, grown to OD600nm≥1.0 at 37°C and induced with 0.4mM IPTG at room temperature to an OD600nm≥1.5.
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Additional flash memory can be added to either of the two models, the $80 IRDC150 and $100 IRDC250, which come with 1GB and 2GB of memory, respectively, giving you either 36 or 72 minutes of video.
For this work, ethanol vapor was added to either the fuel or the oxidizer gases.
For solid plates, 2% agar was added to either YPD or SD medium prior to autoclaving.
Sound files can be added to either overwrite existing audio or mix in with it.
To obtain folate-coated nanoparticles, a folate ligand was added to either the microemulsion templates or nanoparticle suspensions at 25 °C.
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