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Exact(12)
Then, 1.56 mL of ammonium hydroxide solution (30 33 % NH3 in H2O) and 0.15 M of potassium persulfate (K2S2O8) were added subsequently to the mixture.
The extract was dissolved in 7 µL of methanol and then 7 µL of 0.88% aqueous formic acid (v/v) were added, subsequently centrifuged at 3500g for 10 min and the supernatant was injected to the HPLC system.
Following homogenization 750 µl of the homogenate were transferred into a 1.5 mL Eppendorf and 250 µl TCA were added, subsequently the mixture was vortexed thoroughly and centrifuged 10 minutes at 4,000 g.
Primers and templates were added subsequently.
Two additional subjects who suffered from serious adverse events during the study were added subsequently.
PE (Phycoerythrin) conjugated mouse monoclonal antihuman TRAIL or its receptor antibodies were added subsequently to each tube.
Similar(48)
Antibodies in test sera compete with a rabies polyclonal rabbit antiserum which was added subsequently.
About 300 μL of QDs-labeled secondary antibody solution was added subsequently.
Pattern number 52, which defines the default behavior for moving forward, was added subsequently at the end of the training set.
(S -2-Amino-3-phenyl-1-propanol (1): i-a) A mixture of lithium borohydride (0.27 g, 12 mmol) in dry THF (6 mL) waS -2-Amino-3-phenyl-1-propanolS -2-Amino-3-phenyl-1-propanoll) waS -2-Amino-3-phenyl-1-propanol
The bacterium, Micrococcus lysodeikticus (0.75 mg/mL) was suspended in a sodium phosphate buffer (0.1 M, pH 6.24), 200 µl of suspension was placed in each well of 96-well plates and 20 μL of test serum was added subsequently.
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