Sentence examples for were added downstream from inspiring English sources

In addition, 18 nucleotides corresponding to the 5' portion of the AttP1 site were added downstream of LOXP (product A).

In most transformation experiments in filamentous fungi, a terminator is added downstream of the open reading frame.

We also obtained a control vector where a Stop codon was added downstream of the TAP sequence.

The core 222-bp SV40 terminator (ts) fragment was added downstream of the yellow terminator to stabilize it.

For overproduction of Cdc25-GFP-NLS, a DNA fragment encoding SV40 NLS was added downstream of GFP and the cdc25+ gene ORF was cloned.

The second cistron was also expressed when the hairpin was added downstream of the DsRed reporter gene (lanes DsRedH-4.1s-EGFP).

For the α chain construct, a Xma I site was created 17 bp upstream of the ATG cordon of the TCR-Vα2/Jα27 cDNA; and an 11-bp sequence that represents the beginning of the J-C intron was added downstream of the Jα27 segment, followed by an artificial Not I site.

Next, MAR were added upstream and downstream of the reporter cassette to generate the insulated pARE.MAR vector (Fig. 1A).

For primer design, 600 bp of flanking sequence were added upstream and downstream of the insertion coordinates.

By using the aforementioned primers, SalI and SpeI restriction sites were added upstream and downstream of the swollenin-coding region, respectively.

The nucleotide sequences for the Flag-tag and the biotin ligation site (bls) were added upstream and downstream, respectively, of the PK open-reading frame by PCR incorporation of Gateway recombination tags.