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Trackers were added directly to culture supernatants and incubated for 1 h at 37 °C.
Briefly, 10 µl of the reagent were added directly to the culture medium in each well.
The CNTs were added directly to the fillers or coated onto tabular alumina by catalytic chemical vapor deposition (CVD).
Antifungal drugs were added directly to the chambers and the cells were visualized by fluorescence microscopy beginning directly after addition of drugs and for the subsequent 30 minutes.
Anthracene and naphthalene were added directly into MSM in solid state.
The EV71-GFP viruses were added directly into each well without removing the mAb.
Additionally, two questions (numbered eleven and twelve) were added directly addressing the training potential of the device.
Hoechst 33258 (HO) and propidium iodide (PI, both Sigma, St Louis, MO) were added directly to the cells to final concentrations of 5 and 2 mg/ml, respectively.
b For the fed-batch group, the same concentrations of DBF were added directly into the reaction systems at the same time as batch group.
The chemicals (Sigma) were added directly into cell culture.
In some experiments, peptides were added directly to the splenocytes of F1 mice.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com