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Although most endogenous siRNAs were 22 nt in mock-inoculated N. benthamiana, the DCL4-dependent 21-nt siRNAs and DCL2- and DCL3-dependent 24-nt siRNAs were actively produced after virus infection (Figure 3).
As we did not assess the integrity of the BBB, it is not clear whether these immune mediators entered the brain through a leaky BBB and/or if they were actively produced and released locally in the brain.
Although water was regularly exchanged and experimental containers were frequently cleaned to reduce such effects, it is possible that inhibitory substances accumulated at higher densities or were actively produced by animals in response to higher mate encounter rates.
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The hills to the north of the city were riddled with quarries that were actively producing gypsum, the main ingredient of plaster of Paris.
"When I was given a development deal in the early 80s, there were very few female actresses who were actively producing.
All four sites had been abandoned at the time of the operation, but officials said the evidence collected there confirmed that all were actively producing heroin and morphine.
To account for high conc. of the NaCl- materials, craftsmen in Nzuguni, Kitelela and Mahoma Nyika were actively producing table salt from these materials.
The immunoperoxidase technique, which involves the use of specific antibodies for each cell type, showed that macrophages were the predominant cellular constituents but also that fibroblasts, many of which were not identified on plain histological study, were present and were actively producing collagen.
This observation confirmed that the silenced cells surrounding the apoptotic foci were actively producing high level of Wg.
Similarly, HO-1 expression by heme increased the resistance against oxidative cell injury; notably, this protective effect occurred only in cells that were actively producing BR [ 30].
To mark precisely the cells that were actively producing the Wg proliferative signal, we followed the expression of the wg-lacZ transcriptional reporter.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com