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Samples were acquired on a Becton-Dickinson LSRII flow cytometer.
Sixty-min dynamic PET and optical imaging were acquired on a MDA-MB-435 tumor model.
Confocal images were acquired on a Zeiss LSM 780 or LSM 710 laser scanning confocal microscope.
The data were acquired on a BD FACSJazz and analyzed with FlowJo software (v10; Treestar).
Stacks of optical sections were acquired on a Solamere spinning disk confocal system as described above.
Images were acquired on a spinning-disk microscope using a 63× objective.
Samples were acquired on a Fortessa (BD-Bioscience) or sorted on a MoFlo ASTRIOS (Beckman-Coulter).
Brightfield images were acquired on a Zeiss AXIOIMAGER.A2 using AxioVision software.
Sections (~100 nm) were cut and images were acquired on a JEOL 1010 transmission electron microscope.
Stained cells were acquired on a FACSVerse (BD Biosciences, San Diego, CA, USA).
Echo Planner images were acquired on a 1.5-T MR system.
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