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Total polyphenols (TPC) and fractionation of proanthocyanidins were determined.The statistically generated optimum extraction conditions to obtain the highest total polyphenol concentration were a pressure of 80 bar, CO2 flow rate of 6 kg/h and 20% (w/w) co-solvent.
The optimal SFE conditions for 4-nitrotoluene and 3-nitrotoluene were a pressure of 350 atm, temperature of 35 °C, dynamic extraction time of 30 min and modifier volume of 150 μL.
The optimal conditions to obtain the highest flavonoid yield of P. lobata were a pressure of 20.04 MPa, a temperature of 50.24 °C and a co-solvent amount of 181.24 ml.
The GC column used was HP-Plot Q (30 m, D.I. 0.53 mm), and the injection conditions were a pressure of 20.0 psi for 2 min, ventilation flux of 20.0 mL.min−1 after 30 s and injector temperature of 200 °C.
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